SNP Identification Process of Sweet Potato Varieties and Related Wild Ma-terials with Varied Ploidy
The SNP variation in the genome is characterized by abundant quantity and polymorphism.SNP iden-tification plays an important role in exploring genetic diversity,population structure relationship and breeding in crops.Due to the high degree of ploidy complexity and high degree of heterozygosity,it is difficult to develop,ver-ify and detect SNP in polyploid crops.Sweet potato(Ipomoea batatas(L.)Lam.)and its related different polyploidy wild species I.trifida are all from the family of Convolvulaceae,in view of the large ploidy changes within sweet potato species,we selected the diploid,tetraploid and hexaploid I.trifida,as well as the pentaploid and hexaploid I.batatas for reduced-representation genome sequencing using RAD-seq technology.Taking the genome of culti-vated sweet potato'Taizhong6'as a reference,the process of SNP identification in different ploidy materials was established through mapping,sort,PCR marker duplication and variation detection.The results of IQ-TREE analy-sis of SNP markers confirmed that this process has high reliability.The study use sweet potato genome as reference to obtain SNP markers from different ploidy materials and identify the genetic relationship of wild and cultivated sweet potato with different ploidy which help to facilitate the parental selection of breeding and the identification for SNP markers of other polyploid crop plants.
万方数据
维普
