摘要
本研究以姜(Zingiber officinale)为研究对象,利用ISSR分子标记对中国广泛栽培的67份姜种质资源进行了遗传多样性研究.在100条ISSR引物中筛选出8条引物,经验证它们扩增的条带表现出清晰、重复性良好、多态性高的特点.总计获得255个谱带,其中多态性位点高达253个,多态性位点百分率达99.22％.67份姜的观测等位基因数(Na)、有效等位基因数(Ne)、Nei's基因多样性指数(H)和香农信息指数(Ⅰ)的范围分别为 1.909 1～2.000 0、1.379 1～1.626 9、0.239 0～0.360 6、0.378 5～0.536 0,均值分别为 1.988 6、1.525 4、0.310 5和0.472 5.遗传距离范围为0.023 8～0.666 1,平均值为0.392 2.UPGMA聚类结果表明,当遗传系数为0.71时,67份种质可分为7组.这一研究结果为进一步开展姜种质资源的开发和利用提供了科学依据,对于姜的遗传多样性及其在育种领域的应用具有重要的参考价值.
Abstract
This study focused on ginger(Zingiber officinale),and conducted a genetic diversity analysis using IS-SR molecular markers on 67 germplasm resources widely cultivated in China.Eight primers were selected from 100 ISSR primers,and it was proved that the amplified bands showed clear,good repeatability and high polymor-phism.A total of 255 bands were obtained,of which as many as 253 polymorphic loci with 99.22％percent poly-morphic loci were obtained.The observed number of alleles(Na),effective number of alleles(Ne),Nei's gene di-versity index(H),and Shannon's information index(Ⅰ)of 67 ginger samples were 1.909 1 to 12.000 0,1.379 1 to 1.626 9,0.239 0 to 0.360 6,and 0.378 5 to 0.536 0,respectively.And the mean values for these parameters were 1.988 6,1.525 4,0.310 5,and 0.472 5,respectively.The genetic distance ranged from 0.023 8 to 0.666 1,with an average of 0.392 2.UPGMA clustering indicated the partitioning of the 67 germplasms into 7 groups when the ge-netic coefficient was 0.71.These findings provide a scientific foundation for the further development and utiliza-tion of ginger germplasm resources.The study holds significant reference value for understanding the genetic di-versity of ginger and its applications in the field of breeding.
基金项目
台州市科技计划项目(21nya11)
浙江省"三农九方"科技协作计划项目(2024SNJF015)
浙江省第三次全国农作物种质资源普查与收集行动(111821301354052030)
维普
万方数据