Genetic Diversity Analysis of Ginger(Zingiber officinale)Germplasm Re-sources Based on ISSR Molecular Marker
This study focused on ginger(Zingiber officinale),and conducted a genetic diversity analysis using IS-SR molecular markers on 67 germplasm resources widely cultivated in China.Eight primers were selected from 100 ISSR primers,and it was proved that the amplified bands showed clear,good repeatability and high polymor-phism.A total of 255 bands were obtained,of which as many as 253 polymorphic loci with 99.22％percent poly-morphic loci were obtained.The observed number of alleles(Na),effective number of alleles(Ne),Nei's gene di-versity index(H),and Shannon's information index(Ⅰ)of 67 ginger samples were 1.909 1 to 12.000 0,1.379 1 to 1.626 9,0.239 0 to 0.360 6,and 0.378 5 to 0.536 0,respectively.And the mean values for these parameters were 1.988 6,1.525 4,0.310 5,and 0.472 5,respectively.The genetic distance ranged from 0.023 8 to 0.666 1,with an average of 0.392 2.UPGMA clustering indicated the partitioning of the 67 germplasms into 7 groups when the ge-netic coefficient was 0.71.These findings provide a scientific foundation for the further development and utiliza-tion of ginger germplasm resources.The study holds significant reference value for understanding the genetic di-versity of ginger and its applications in the field of breeding.
万方数据
维普
