Isolation and Identification of Endophytes Derived from Small-flowered Water Lily and Evaluation of Antibiotic Effects
This study aimed to isolate and screen beneficial endophytic fungi from the tropical waterlily,the endo-phytes inhabiting leaves,stems and petals of Nymphaea micrantha Guill.& Perr.were isolated by the water agar method.The obtained strains were identified with ITS sequence analysis methods.The method of constructing phylogenetic trees was used to determine the classification status of strains,antibacterial and antifungal tests were carried out by the drug-sensitive paper sheet method.In this study,a total of 20 endophytes was isolated and screened,including 11 strains of fungi and 9 strains of bacteria.The identification of the selected endophytes yield-ed 17 strains of normal sequencing,which were clearly clustered into two major branches in the phylogenetic tree,and both strains with highly similar relatives were found,and the genus of the strains was determined.8 strains of endophytic fungi were selected for antibiotic experiments,which had certain inhibitory effects on different pathogens.Among them,F-1(Chaetomium globosum),F-3(Diaporthe ueckerae)and S-1(Gymnascella sp.)dis-played strong activity and a wide range antibiotic spectrum.In this study,most of the endophytic fungi obtained exhibit activity,and the endophytic bacteria of Nymphaea micrantha have great potential to develop new antibacte-rial inoculants,which provides basis and reference for the development and utilization of them.
万方数据
维普
