In order to study the genetic diversity of different provenances of Paris polyphylla var.chinensis,40 pro-venances of P.polyphylla var.chinensis were sequenced and analyzed using ITS and psbA-trnH sequence primers,and a phylogenetic tree was constructed using MEGA 5.0 software.The results showed as followings:the total lengths of ITS sequences were 634 bp and 635 bp,the lengths of three fragments of ITS 1,5.8S and ITS2 were 241～242 bp,165 bp and 228 bp,respectively,and there were 21 variable sites among the sequences;however,the total lengths of psbA-trnH sequence were between 1 024 and 1 044 bp and contained 33 variable sites.The genetic dis-tance of the ITS sequence among different provenances was between 0 and 0.014 2,and its average genetic dis-tance is 0.003 4;while,the genetic distance of psbA-trnH sequence was between 0 and 0.012 7,and its average ge-netic distance was 0.002 3.The clustering results showed that the genetic relationship of P.polyphylla var.chinen-sis among different provenances had some correlation with its geographical distribution under the ITS sequence to a certain degree,however,there was no obvious correlation under the psbA-trnH sequences.The results also showed that the genetic diversity of the ITS sequencesamong different provenances was relatively richer than ps-bA-trnH,and the ITS sequence was more suitable for the study of genetic diversity within the P.polyphylla var.chinensis species.Therefore,the results provide a theoretical basis for the molecular identification and genetic di-versity analysis of P.polyphylla var.chinensis.
关键词
华重楼/ITS序列/psbA-trnH序列/遗传多样性/聚类分析
Key words
Paris polyphylla var.chinensis/ITS sequence/psbA-trnH sequence/Genetic diversity/Cluster analysis
维普
万方数据