Effects Analysis of Exogenous Proline Application on Alleviating Freezing Injury of Winter Wheat at Jointing Stage
Winter wheat is extremely sensitive to low temperature during the spring jointing stage.In recent years,wheat freezing damage has occurred frequently in spring,the yield of damaged wheat reduced by 10％to 30％,and the serious ones can reach more than 50％.In the practice of wheat production,there is an urgent need for an-ti-stress reagents that can reduce or protect wheat from freezing damage in spring.Based on this,this study ex-plored the effect of exogenous application of proline on the freezing resistance of winter wheat at the jointing stage.In this research,experiment applied in pot culture,winter wheat variety'Bainong207'was used as material,and chamber to simulate freezing stress with artificial climate at the jointing stage.Then wheat applied at a con-centration of 20 mmol/L with proline was screened for better freezing resistance under the low temperature stress.Measured the content of the relative expression levels of various physiological indexes and stress resistance related genes,were used to verify the effect of exogenous proline on the freezing resistance of wheat.The results showed that exogenous application of 20 mmol/L proline could significantly decrease the content of malondialdehyde(MDA)and relative conductivity,and increase the content of free proline(Pro)in wheat leaves under low tempera-ture treatment.Exogenous proline could increase the expression of SOD,POD and CA T genes in wheat leaves un-der low temperature stress,the relative expression levels of WCS120,P5CS and LEA genes were significantly high-er than those of the control at 0 h and 24 h after low temperature treatment;moreover,the freezing death rate of wheat young spike was significantly reduced by exogenous application of 20 mmol/L proline.Therefore,applying the 20 mmol/L proline of winter wheat before the low temperature at the jointing stage can effectively alleviate the damage caused by low temperature to winter wheat and improve the freezing resistance of wheat.
Winter wheatExogenous prolineLow temperature stressRelative gene expression
万方数据
维普
