Cloning and Expression Pattern Analysis of Maize AINTEG UMENTA-LIKE 1(AIL1)Gene
To understand the role of ZmAIL1,a member of the euANT subfamily of the AP2/ERF transcription factor family,in maize growth and development,the full-length cDNA of ZmAIL1 gene was cloned using RT-PCR with maize'Xianyu335'as the experimental material.Bioinformatics analysis was conducted,and its expression pattern in different tissues and under abiotic stress conditions was examined by real-time quantitative PCR.The results showed that the cDNA length of ZmAIL1 was 1 273 bp,containing an 873 bp open reading frame and encoding 290 amino acids,with a relative molecular weight is 27.258 kD and a theoretical isoelectric point of 8.69,and was a hydrophobic protein.Phylogenetic analysis showed that it was closely related to Sorghum bicolor and other gramineous plants.RT-qPCR analysis showed that it was mainly expressed in leaves and tassels,and the expression was induced under different abiotic stress conditions.Moreover,the expression levels of ZmA IL1 were significantly up-regulated in seedling leaf tissues under salt and drought stress.These results provide a scientific basis for further exploring the function of ZmAIL1 gene.
万方数据
维普
