Gene Expression Analysis of Exogenous CPPU Inhibition the Coloration Process of'Feizixiao'Litchi Fruit
In order to explore the relationship between the anthocyanin and chlorophyll related structural genes with the coloration of'Feizixiao'litchi,identification of the above gene based on genome data was conducted firstly,and then analyzed their expression and corresponding enzyme activities during the coloration inhibition of'Feizixiao'litchi induced by exogenous CPPU in this study.The results showed that the key enzyme UFGT in anthocyanin biosynthesis,as well as the enzymes NYC/NOL,MDcase,and PAO involved in the chlorophyll degradation path way,were significantly inhibited,while the activities of CS and CAO in the chlorophyll synthesis pathway,along with Chlase,were notably increased.Transcriptome sequencing and RT-qPCR results indicated that exogenous CPPU significantly inhibited the expression of structural genes involved in the anthocyanin biosynthesis pathway,includ-ing LcPAL1,LcPAL3,LcC4H1,Lc4CL2,Lc4CL3,Lc4CL4,LcCHS2,LcCHS5,LcCHI,LcF3H/LcF3'H,LcDFR2,LcANS1,LcUFGT2,LcUFGT3,and those involved in the chlorophyll degradation pathway,including LcSGR2,LcPPH,and LcPA O.At the same time,it promoted the expression of genes related to chlorophyll synthesis,such as LcHEMA,LcHEMD,Lc CHLH1,Lc CHLH2 and LcCAO,while inhibiting the expression of transcription factor genes LcMYB3,LcMYB4 and LcWRKY3.Therefore,it is speculated the expression and enzyme activity of genes related to anthocyanin synthesis and chlorophyll degradation may be inhibited,the expression and enzyme activity of genes related to chlorophyll synthesis may be promoted by exogenous CPPU to make the ratio of anthocyanin to chloro-phyll in the pericarp of'Feizixiao'litchi fruit lower in the best edible period and unable to complete coloration.The results provide a theoretical basis for solving the problem of"stay green"in'Feizixiao'litchi,and also provide a ref-erence for the study of plant color.
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