Camellia nitidissima is endemic plant in China.It is precious germplasm resource for cultivating yel-low camellia,which has important ornamental value and medicinal value.Full-length transcriptome sequencing data of Camellia nitidissima can help to find genes which regulate the synthesis of flower color,but the full-length Camellia nitidissima transcriptome is still lacking.So the alabastrum,flower bud and blooming flowers mixed e-qually were sequenced using single-molecule real-time sequencing technology to generate the transcriptome of Camellia nitidissima.The sequencing results showed that 179 645 full-length non-contatemer(FLNC)were symbi-otic in 28.2 Gb raw data,from which 45 372 high-quality Isoforms were obtained.7 752 alternative splicing events and 1 730 long non-coding RNA sequences were identified through prediction of alternative splicing and long non-coding RNA.43 348,36 909,29 452 and 42 163 Isoforms were respectively assigned to the NR,Swiss-Prot,KOG and KEGG databases in gene annotation analysis,and 1 787 transcription factors were detected.The results of Gene Ontology annotation and KEGG analysis indicated the gene expression profile of alabastrum,flower bud and blooming flowers was mainly related to the synthesis of secondary metabolites,cell functions and cell compo-nents.This study provided a large number of full-length transcript sequences,which will lay a foundation for the molecular biology and genetics of Camellia nitidissima in the future.
维普
万方数据