基于单分子实时测序的金花茶全长转录组分析

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维普

中文摘要：金花茶是中国特有植物，是培养黄色山茶花的珍贵种质资源，具有重要的观赏价值和药用价值。金花茶全长转录组测序数据有助于筛选出调控花色合成的基因，但是金花茶的全长转录组数据仍然缺乏。因此，本研究利用单分子实时测序技术对金花茶花芽、花蕾以及盛开的花进行混合样品的全长转录组测序。测序结果表明，在28。2 Gb的原始测序数据中，共生成了 179 645个全长非嵌合序列(FLNC)，从中获得了 45 372个高质量的全长转录本(Isoform)。对获得的全长转录本进行可变剪切分析及长链非编码RNA预测，鉴定出了 7 752个可变剪接事件和1 730个长链非编码RNA序列;在基因注释分析中，NR、Swiss-Prot、KOG和KEGG数据库分别注释了 43 348、36 909、29452和42 163条转录本，检测到了 1 787个转录因子。GO注释及KEGG代谢通路分析表明，金花茶的花芽、花蕾、开放花朵等组织的基因表达谱主要与次生代谢物质合成、细胞功能以及细胞组分合成有关。本研究提供了大量的全长转录本序列，为今后金花茶的分子生物学和遗传学研究奠定了基础。

外文标题：Full-length Transcriptome Analysis of Camellia nitidis sima Based on Sin-gle-Molecule Real-Time Sequencing Technology

外文摘要：Camellia nitidissima is endemic plant in China.It is precious germplasm resource for cultivating yel-low camellia,which has important ornamental value and medicinal value.Full-length transcriptome sequencing data of Camellia nitidissima can help to find genes which regulate the synthesis of flower color,but the full-length Camellia nitidissima transcriptome is still lacking.So the alabastrum,flower bud and blooming flowers mixed e-qually were sequenced using single-molecule real-time sequencing technology to generate the transcriptome of Camellia nitidissima.The sequencing results showed that 179 645 full-length non-contatemer(FLNC)were symbi-otic in 28.2 Gb raw data,from which 45 372 high-quality Isoforms were obtained.7 752 alternative splicing events and 1 730 long non-coding RNA sequences were identified through prediction of alternative splicing and long non-coding RNA.43 348,36 909,29 452 and 42 163 Isoforms were respectively assigned to the NR,Swiss-Prot,KOG and KEGG databases in gene annotation analysis,and 1 787 transcription factors were detected.The results of Gene Ontology annotation and KEGG analysis indicated the gene expression profile of alabastrum,flower bud and blooming flowers was mainly related to the synthesis of secondary metabolites,cell functions and cell compo-nents.This study provided a large number of full-length transcript sequences,which will lay a foundation for the molecular biology and genetics of Camellia nitidissima in the future.

外文关键词：

Camellia nitidissimaThe single-molecule real-time sequencing technologyAlternative splicingLong non-coding RNA

作者：

刘合霞、姚金双、刘雨、谢心怡、耿晓姗、李博、周兴文

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作者单位：

玉林师范学院生物与制药学院,玉林,537000

福建工程学院建筑与城乡规划学院,福州,350108

关键词：

金花茶单分子实时测序可变剪切长非编码RNA

基金：

玉林师范学院高层次人才科研启动基金项目玉林师范学院高层次人才科研启动基金项目玉林师范学院高层次人才科研启动基金项目玉林师范学院大学生创新创业训练计划项目玉林市科学研究与技术开发计划项目广西高校中青年教师科研基础能力提升项目广西自然科学基金重点项目国家自然科学基金项目

项目编号：

G2018025G2019ZK13G2019ZK35202010606164玉市科能201943032020KY140122018GXNSFDA28100731860228

出版年：

2024

DOI：

10.13271/j.mpb.022.007031

分子植物育种

海南省生物工程协会

分子植物育种

CSTPCD北大核心

影响因子：0.765

ISSN：1672-416X

年,卷(期)：2024.22(21)