Constructing Cucumber Fingerprints Based on SNP Molecular Markers
Cucumber is one of the most widely cultivated,produced,and consumed vegetable crops in the world,with high economic value.In order to strengthen the protection and utilization of cucumber germplasm resources,this experiment used resequencing data from 259 cucumber materials for SNP identification.The SNP set was screened based on indicators such as site deletion rate,heterozygosity,and polymorphism information content.Finally,150 core SNP markers that can efficiently identify cucumber germplasm were obtained.The average PIC and MAF values of the core SNP site combination in this set are 0.374 and 0.479,respectively.Through cluster analysis,it can be con-cluded that 259 cucumber germplasms can be divided into two major groups:East Asian type and Eurasian type.A DNA fingerprint map was constructed for each cucumber germplasm based on the core SNP markers mentioned above,which can effectively distinguish all the tested materials.This study provides important theoretical basis for genetic diversity analysis,germplasm resource identification,and new variety cultivation of cucumber.