Selection of Optimal Reference Genes for Quantitative Real-time PCR in Catalpa bungei under Different Stress Conditions
Catalpa bungei C.A.Meyer.is a precious and unique tree species in China,with high material,medici-nal and ornamental values,but there are few reports on its functional genes.In order to provide a more accurate reference for the quantitative experimental results of subsequent expression analysis of related functional genes in Catalpa bungei,it is necessary to screen genes with high expression stability as internal reference genes.In this study,based on the transcriptome data from Catalpa bungei,ten housekeeping genes,including CYP40-1,CYP40-2,EF1 A,EF1B,MDH,PP2A,TUBA,TUBB,UBC2 and H3-1,were selected as candidate internal reference genes.Then,the cultured Catalpa bungei seedlings were treated by low temperature,high temperature,drought and high salinity,re-spectively,and then the expression levels of ten candidate genes were monitored by quantitative real-time PCR.Fi-nally,analysis of the gene expression stability by three methods(geNorm,Normfinder and BestKeeper)suggested that UBC2,CYP40-2,PP2A and CYP40-1 were the best reference genes under 4 ℃,37 ℃,300 mmol/L mannitol,and 100 mmol/L NaCl,respectively.The most stable internal reference genes and the corresponding primer se-quences provided in this study will facilitate further gene expression analysis in Catalpa bungei under different stress conditions.
万方数据
维普
