分子植物育种2024,Vol.22Issue(21) :7102-7109.DOI:10.13271/j.mpb.022.007102

黄淮麦区(南片)小麦高分子量麦谷蛋白亚基组成分析

Composition Analysis of High Molecular Weight Glutenin Subunits of Wheat in Huanghuai Southern Wheat Region

陈杰 朱保磊 白冬 杨一帆 宋佳静 宋全昊 赵立尚 朱统泉 梁宝萍 宋晓朋
分子植物育种2024,Vol.22Issue(21) :7102-7109.DOI:10.13271/j.mpb.022.007102

黄淮麦区(南片)小麦高分子量麦谷蛋白亚基组成分析

Composition Analysis of High Molecular Weight Glutenin Subunits of Wheat in Huanghuai Southern Wheat Region

陈杰 1朱保磊 2白冬 1杨一帆 3宋佳静 1宋全昊 1赵立尚 1朱统泉 1梁宝萍 1宋晓朋1
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作者信息

  • 1. 驻马店市农业科学院,驻马店,463000
  • 2. 信阳市农业科学院,信阳,464000
  • 3. 驻马店市农业科学试验站,驻马店,463000
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摘要

为了解高分子量麦谷蛋白亚基(HMW-GS)在当前黄淮麦区(南片)小麦品种中的分布情况,本研究以94个黄淮麦区(南片)新育成的小麦品种为供试材料,利用SDS-PAGE技术对供试材料高分子量麦谷蛋白亚基(HMW-GS)进行鉴定,并探讨了这些亚基和亚基组合与蛋白质含量之间的关系.结果表明,在Glu-A1位点上,亚基1和N在供试材料中的分布频率分别为72.34%和27.66%,与高蛋白质含量相关的亚基1分布频率较高;在Glu-B1位点上,亚基14+15、7+8和7+9的分布频率分别为9.57%、51.06%和39.36%,以与高蛋白质含量相关的亚基7+8分布频率较高;在Glu-D1位点上,亚基2+12和5+10的分布频率分别为48.94%和51.06%,以与高蛋白质含量相关的亚基5+10分布频率较高.在Glu-A1、Glu-B1和Glu-D1位点上,共检测到12种亚基组合类型,其中与高蛋白质含量相关的亚基组合1/7+8/5+10分布频率为25.53%,与低蛋白质含量相关的亚基组合N/7+9/2+12分布频率为13.83%,其余10种亚基组合的分布频率合计为60.64%.本研究能够为黄淮麦区(南片)小麦品质改良提供参考信息.

Abstract

In order to understand the distribution of high molecular weight glutenin subunits(HMW-GS)in wheat varieties from Huanghuai southern region.In this study,a total of 94 wheat varieties newly cultivated in the Huanghuai southern region were used as the test materials.The high molecular weight glutenin subunits(HMW-GS)were identified by SDS-PAGE,and the relationship between these subunits and protein content was discussed.The results showed that in the Glu-A1 locus,the distribution frequencies of subunit 1 and N in the test materials were 72.34%and 27.66%respectively,and the distribution frequency of subunit 1 related to high protein content was higher;in the Glu-B1 locus,the distribution frequencies of subunit 14+15,7+8,and 7+9 in the test materials were 9.57%,51.06%,and 39.36%respectively,and the distribution frequency of subunit 7+8 related to high pro-tein content was higher;in the Glu-D1 locus,the distribution frequencies of subunit 2+12 and 5+10 in the test ma-terials were 48.94%and 51.06%respectively,and the distribution frequency of subunit 5+10 related to high pro-tein content was higher.A total of 12 subunit combinations were detected in Glu-A1,Glu-B1 and Glu-D1 locus.The distribution frequency of the subunit combination 1/7+8/5+10 related to high protein content was 25.53%,the distribution frequency of the subunit combination N/7+9/2+12 related to low protein content was 13.83%,and the total distribution frequency of the remaining 10 subunit combinations was 60.64%.This study can provide reference information for wheat quality improvement in Huanghuai southern region.

关键词

黄淮麦区/小麦/高分子量麦谷蛋白亚基/蛋白质含量

Key words

Huanghuai wheat region/Wheat/HMW-GS/Protein content

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基金项目

国家现代农业产业技术体系项目(CARS-03)

河南省小麦重大科技专项(151100110400)

驻马店市科技攻关项目(14103)

驻马店市科技攻关项目(15124)

驻马店市科技攻关项目(16108)

驻马店市科技攻关项目(17137)

出版年

2024
分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
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