Composition Analysis of High Molecular Weight Glutenin Subunits of Wheat in Huanghuai Southern Wheat Region
In order to understand the distribution of high molecular weight glutenin subunits(HMW-GS)in wheat varieties from Huanghuai southern region.In this study,a total of 94 wheat varieties newly cultivated in the Huanghuai southern region were used as the test materials.The high molecular weight glutenin subunits(HMW-GS)were identified by SDS-PAGE,and the relationship between these subunits and protein content was discussed.The results showed that in the Glu-A1 locus,the distribution frequencies of subunit 1 and N in the test materials were 72.34%and 27.66%respectively,and the distribution frequency of subunit 1 related to high protein content was higher;in the Glu-B1 locus,the distribution frequencies of subunit 14+15,7+8,and 7+9 in the test materials were 9.57%,51.06%,and 39.36%respectively,and the distribution frequency of subunit 7+8 related to high pro-tein content was higher;in the Glu-D1 locus,the distribution frequencies of subunit 2+12 and 5+10 in the test ma-terials were 48.94%and 51.06%respectively,and the distribution frequency of subunit 5+10 related to high pro-tein content was higher.A total of 12 subunit combinations were detected in Glu-A1,Glu-B1 and Glu-D1 locus.The distribution frequency of the subunit combination 1/7+8/5+10 related to high protein content was 25.53%,the distribution frequency of the subunit combination N/7+9/2+12 related to low protein content was 13.83%,and the total distribution frequency of the remaining 10 subunit combinations was 60.64%.This study can provide reference information for wheat quality improvement in Huanghuai southern region.