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UPLC-MS/MS测定三药槟榔B族维生素含量及相关性分析

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为了更好地了解三药槟榔(Areca triandra Roxb.)不同组织中的B族维生素的动态变化,本研究通过超高液相色谱-三重四极杆串联质谱(UPLC-MS/MS)技术,对三药槟榔的4个不同成熟阶段的果实的果皮和胚乳、不同成熟阶段的叶片组织、雌雄花和花序进行了 B族维生素含量的检测与分析.结果显示,三药槟榔中检测到9种水溶性B族维生素(包括硫胺素,核黄素,烟酸,烟酰胺,泛酸,吡哆醇,生物素,叶酸,氰钴胺素).相同维生素在不同组织中的含量有较大差异.三药槟榔各组织中最为丰富的B族维生素是硫胺素和泛酸,不同组织及不同发育阶段B族维生素的含量呈现出不同的动态变化.B族维生素在三药槟榔中的分布具有组织特异性和成熟度依赖性的特点.不同B族维生素在三药槟榔果皮和胚乳的发育过程中呈现出不同的积累模式.此外,相关性分析发现,硫胺素与烟酸呈现显著正相关,吡哆醇与氰钴胺素呈现显著正相关,核黄素与生物素呈现显著正相关.本研究为探索三药槟榔不同组织中B族维生素的含量以及果实和叶片的不同发育阶段B族维生素含量的动态积累模式提供了参考.
Determination and Correlation Analysis of B Vitamins Content in Areca triandra by UPLCMS/MS
To better understand the role of the dynamics of B vitamins in different tissues in Areca triandra,this study determined and analysed the content of B vitamins in the four development stages of endosperm and peri-carp,leaf tissue at different stages of maturity,male and female flowers and inflorescences of the A.triandra by ultra-high liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS).The results showed that nine water-soluble B vitamins(including thiamin,riboflavin,niacin,nicotinamide,pantothenic acid,pyridox-ine,biotin,folic acid and cyanocobalamin)were detected in the A.triandra.The amount of the same vitamin varies considerably in different tissues.The most abundant B vitamins in the tissues of the A.triandra are thiamine and pantothenic acid.The content of B vitamins showed different dynamics in different tissues and developmental stages.The distribution of B vitamins in A.triandra has the characteristics of tissue specificity and maturity depen-dence.Different B vitamins showed different accumulation patterns during the development of the pericarp and en-dosperm of A.triandra.In addition,correlation analysis found that thiamine was significantly positively correlated with niacin,pyridoxine was significantly positively correlated with cyanocobalanim,and riboflavin was significantly positively correlated with biotin.This study provides a reference for exploring the contents of B vitamins in differ-ent tissues of the A.triandra and the dynamic accumulation patterns of B vitamins content in fruits and leaves at different developmental stages.

Ultra-high liquid chromatography-triple quadrupole tandem mass spectrometryAreca triandraContent of B vitaminsCorrelation analysis

蒋雯秀、吴娇、周广振、万迎朗

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海南大学热带作物学院,海口,570228

中国热带农业科学院环境与植物保护研究所,海口,571101

超高液相色谱-三重四极杆串联质谱 三药槟榔 B族维生素含量 相关性分析

海南省科技计划项目

ZDYF2021XDNY121

2024

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCD北大核心
影响因子:0.765
ISSN:1672-416X
年,卷(期):2024.22(21)