In this study,artificial rainfall simulation was used to study the long-term and short-term drought stress of annual potted A tractylodes chinensis;the content of atractylodin in rhizomes of A tractylodes chinensis under different drought stress was determined by high performance liquid chromatography;the effects of different drought stress on the activity of ACC,the key enzyme of atractylodin synthesis,were measured by double antibody sand-wich method;the gene expression of key enzyme ACC under different drought stress was determined by real-time fluorescence quantitative PCR(RT-qPCR).The results showed that during the reproductive growth period,atracty-lodin accumulated significantly under light and moderate drought stress,and the content of atractylodes was higher than CK increased by 9.67％and 10.63％(long-term),in the short-term drought stress treatment 12 d atractylodin content of peak,6.36％higher than that of CK.Severe drought stress significantly inhibited the synthesis of atracty-lodin.Long-term mild,moderate and short-term drought stress was beneficial to significantly increase the enzyme activity and gene expression of ACC(P＜0.05).Long term severe drought stress inhibited the enzyme activity and expression of ACC in atractylodin biosynthesis pathway,which decreased by 12.08％and 48.66％respectively compared with CK,and then significantly inhibited atractylodin synthesis.This study has important theoretical value for the quality control of A tractylodes chinensis.
维普
万方数据