Establishment and Propagation Analysis of Grass Coral Regeneration Sys-tem
In order to establish the regeneration system of grass coral(Sarcandra glabra),speed up its reproduction rate,shorten the breeding cycle,and provide certain reference for the tissue culture and industrial development and utilization of grass coral,this study took grass coral stem segment as explants and controlled the sampling time of explants,the types and duration of disinfection reagents,the types of culture medium and the concentration ratio of exogenous hormones.From primary culture to secondary culture to sprout,strong seedling and root and tube seedling transplanting,so as to obtain the best culture conditions.The results showed that the best time to pick the explants was from March to May.Axillary bud stem segments were treated with 2％NaClO for 14 minutes or 0.1％HgCl2 for 12 minutes to achieve the best disinfection effect.The bud induction medium was MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.5％sucrose+1.0 g/L activated carbon+0.7％AGAR,and the germination rate could reach 85％.The proliferation medium was MS+1.0 mg/L 6-BA+2.5％sucrose+1.0 g/L activated carbon+0.7％AGAR,and the proliferation coefficient could reach 5.9.The root induction medium was 1/2MS+1.0 mg/L IBA+0.1 mg/L NAA+2.5％sucrose+1.0 g/L activated carbon+0.7％AGAR,the rooting rate reached 95％.The survival rate of grass coral seedlings was the highest when sand:humus 1:1 was used as the substrate.The aseptic regeneration sys-tem established in this study can provide some reference for the industrial production of grass coral.
万方数据
维普
