Whole Genome Survey Analysis of Trollius chinensis
In order to reveal the genome size of Trollius chinensis,the genome suvery was analyzed with Illumina NovaSeq sequencing platform and bioinformatics methods in this study.The results showed that the genome size of T.chinensis was about 3.96 Gb,with 1.66％heterozygosity rate,74.14％repeats and 40.04％GC content.In the genome sequence,52 907 simple sequence repeats(SSRs)were detected,among which the tri-nucleotide repetitive repeats accounted for the highest rate,up to 28.23％.Ten thousand reads were randomly extracted from the se-quencing data for blastn comparison with NT database.The results showed that 2 867 reads could be matched to entries in the database,with the majority of hits classified as Ranunculoideae,comprising 656 reads.The results of genome survey showed that the genome of T.chinensis was large and complicated,and the high quality genome would be obtained based on the combination the second generation(Illumina)and third generation(PacBio)se-quencing technology.This study preliminarily explored the size and basic characteristics of the genome in T.chi-nensis,providing a reference basis for its genome sequencing research in the future.
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