SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Muscle Regeneration

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Skeletal muscle has a robust regeneration ability that is impaired by severe injury,disease,and aging,resulting in a decline in skeletal muscle function.Therefore,improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders.Owing to their significant role in tissue regeneration,implantation of M2 macrophages(M2Mo)has great potential for improving skeletal muscle regeneration.Here,we present a short-wave infrared(SWIR)fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mo transplantation.SWIR fluorescence imaging was employed to track implanted M2Mo in the injured skeletal muscle of mouse models.It is found that the implanted M2Me accumulated at the injury site for two weeks.Then,SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5(1.09±0.09 vs 0.85±0.05;p=0.01)and day 9(1.38±0.16 vs 0.95±0.03;p=0.01)post-injury,as well as augment the degree of skeletal muscle regen-eration on day 13 post-injury.Finally,multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regen-eration.These results provide more in vivo details about M2Mo in skeletal muscle regeneration and con-firm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair,which will guide the research and development of M2Mo implantation to improve skeletal muscle regeneration.

外文关键词：

In vivoShort-wave infraredSkeletal muscleMacrophageRegeneration

作者：

Mo Chen、Yuzhou Chen、Sijia Feng、Shixian Dong、Luyi Sun、Huizhu Li、Fuchun Chen、Nguyen Thi Kim Thanh、Yunxia Li、Shiyi Chen、You Wang、Jun Chen

展开 >

作者单位：

Sports Medicine Institute of Fudan University,Department of Sports Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China

Department of Bone and Joint Surgery,Department of Orthopedics,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China

Department of Anatomy and Physiology,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China

Key Laboratory of Infrared System Detection & Imaging Technology,Shanghai Institute of Technical Physics,Chinese Academy of Sciences,Shanghai 200083,China

Biophysics Group,Department of Physics and Astronomy,University College London,London WC1E 6BT,UK

Healthcare Biomagnetics and Nanomaterials Laboratories,University College London,London W1S 4BS,UK

展开 >

基金：

Shanghai Sailing Program国家重点研发计划国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金Sanming Project of Medicine in ShenzhenIntroduction Project of Clinical Medicine Expert Team for SuzhouShanghai Talent Development Funding SchemeShanghai Sailing ProgramShanghai Sailing ProgramResearch Project of Shanghai Science and Technology Commission

项目编号：

22YF14387002021YFA1201303821725118197212181972129820725218201153002382111530200SZSM201612078SZYJTD201714202008021YF140410022YF140520022DZ2204900

出版年：

2024

DOI：

10.1016/j.eng.2023.05.010

工程(英文)

CSTPCDEI

ISSN：2095-8099

年,卷(期)：2024.33(2)

参考文献量38