Rational Design of and Mechanism Insight into an Efficient Antifreeze Peptide for Cryopreservation

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外文摘要：The development of effective antifreeze peptides to control ice growth has attracted a significant amount of attention yet still remains a great challenge.Here,we propose a novel design method based on in-depth investigation of repetitive motifs in various ice-binding proteins(IBPs)with evolution analysis.In this way,several peptides with notable antifreeze activity were developed.In particular,a designed antifreeze peptide named AVD exhibits ideal ice recrystallization inhibition(IRI),solubility,and biocom-patibility,making it suitable for use as a cryoprotective agent(CPA).A mutation analysis and molecular dynamics(MD)simulations indicated that the Thr6 and Asn8 residues of the AVD peptide are fundamen-tal to its ice-binding capacity,while the Seri 8 residue can synergistically enhance their interaction with ice,revealing the antifreeze mechanism of AVD.Furthermore,to evaluate the cryoprotection potential of AVD,the peptide was successfully employed for the cryopreservation of various cells,which demon-strated significant post-freezing cell recovery.This work opens up a new avenue for designing antifreeze materials and provides peptide-based functional modules for synthetic biology.

外文关键词：

Antifreeze peptidesEvolution analysisIce recrystallization inhibitionMolecular dynamics simulationCryopreservationSynthetic biology

作者：

Haishan Qi、Yihang Gao、Lin Zhang、Zhongxin Cui、Xiaojie Sui、Jianfan Ma、Jing Yang、Zhiquan Shu、Lei Zhang

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作者单位：

Department of Biochemical Engineering,School of Chemical Engineering and Technology,Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE),Tianjin University,Tianjin 300350,China

School of Engineering and Technology,University of Washington Tacoma,Tacoma,WA 98402,USA

基金：

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaOpen Funding Project of the National Key Laboratory of Biochemical EngineeringProgram of Introducing Talents of Discipline to Universities

项目编号：

2021YFC2100800220782382196113200521908160BP0618007

出版年：

2024

DOI：

10.1016/j.eng.2023.01.015

工程(英文)

CSTPCDEI

ISSN：2095-8099

年,卷(期)：2024.34(3)