Biomimetic Macrophage-Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichia coli

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外文摘要：Infections with multidrug-resistant(MDR)Gram-negative bacteria,such as MDR Escherichia coli(E.coli),remain a challenge due to the lack of safe antibiotics and high fatality rates under anti-infection thera-pies.This work presents a form of biomimetic intelligent catalysis inspired by the selective biocatalytic property of macrophages(MΦs),consisting of an intelligent controlling center(a living MΦ)and a Fenton reaction catalyst(Fe3O4@poly(lactic-co-glycolic acid)(PLGA)nanoparticles)for killing MDR E.coli with-out harming normal cells.The MΦ-Fe3O4@PLGA particles(i.e.,the intelligent catalysis particles)exhibit selective biocatalysis activity toward MDR E.coli by producing H2O2 and lipid droplets(LDs).This process activates the lipid metabolism and glycan biosynthesis and metabolism pathways based on the result of RNA sequencing data analysis.The H2O2 further reacts with Fe3O4@PLGA to form highly toxic hydroxyl radicals(·OH),while the LDs contain antimicrobial peptides and can target MDR E.coli.The highly toxic·OH and antimicrobial peptides are shown to combat with MDR E.coli,such that the antibacterial effi-ciency of the MΦ-Fe3O4@PLGA particles against MDR E.coli is 99.29％±0.31％in vitro.More importantly,after several passages,the intelligent catalysis function of the MΦ-Fe3O4@PLGA particles is well main-tained.Hence,the concept of biomimetic intelligent catalysts displays potential for treating diseases other than infections.

外文关键词：

Multidrug-resistant Escherichia coli Macrophage-Fe3O4@PLGA particlesBiomimetic intelligent catalysis

作者：

Jieni Fu、Xiangmei Liu、Zhaoyang Li、Yufeng Zheng、Yu Zhang、Hui Jiang、Yanqin Liang、Shengli Zhu、Zhenduo Cui、Shuilin Wu

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作者单位：

Key Laboratory of Advanced Ceramics and Machining Technology of the Ministry of Education,School of Materials Science and Engineering,Tianjin University,Tianjin 300401,China

School of Materials Science and Engineering,Peking University,Beijing 100871,China

School of Health Sciences and Biomedical Engineering,Hebei University of Technology,Tianjin 300401,China

School of Materials Science and Engineering Peking University,Beijing 100871,China

Department of Orthopedics,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China

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基金：

China National Funds for Distinguished Young ScientistsNational Natural Science Foundation of China(NSFC)National Natural Science Foundation of China(NSFC)NSFC-Guangdong Province Joint ProgramCentral Guidance on Local Science and Technology Development Fund of Hebei Province

项目编号：

519251045187116252173251U21A2084226Z1303G

出版年：

2024

DOI：

10.1016/j.eng.2023.05.022

工程(英文)

CSTPCDEI

ISSN：2095-8099

年,卷(期)：2024.34(3)