首页|A Double-Layer Polysaccharide Hydrogel(DPH)for the Enhanced Intestine-Targeted Oral Delivery of Probiotics
A Double-Layer Polysaccharide Hydrogel(DPH)for the Enhanced Intestine-Targeted Oral Delivery of Probiotics
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Transplantation of probiotics to the intestine can positively regulate the gut microbiota,thereby promot-ing the immune system and treating various diseases.However,the harsh gastrointestinal environment and short retention time in the gastrointestinal tract significantly limit the bioavailability and intestinal colonization of probiotics.Herein,we present a double-layer polysaccharide hydrogel(DPH)in the form of a double-layer structure composed of a carboxymethyl cellulose(CMCL)supramolecular inner layer and a dialdehyde alginate(DAA)cross-linked carboxymethyl chitosan(CMCS)outer layer.This double-layer structure allows DPH to encapsulate and deliver probiotics in a targeted manner within the body.In the stomach,the cage structure of the DPH is closed,and the outer layer absorbs surrounding liquids to form a barrier to protect the probiotics from gastric fluids.In the intestine,the cage structure opens and disintegrates,releasing the probiotics.Thus,DPH endows probiotics with excellent intestine-targeted delivery,improved oral bioavailability,enhanced gastrointestinal tract tolerance,and robust mucoadhe-sion capacity.The encapsulated probiotics exhibit almost unchanged bioactivity in the gastrointestinal tract before release,as well as improved oral delivery.In particular,probiotics encapsulated by DPH exhi-bit 100.1 times higher bioavailability and 10.6 times higher mucoadhesion than free probiotics in an ani-mal model 48 h post-treatment.In addition,with a remarkable ability to survive and be retained in the intestine,probiotics encapsulated by DPH show excellent in vitro and in vivo competition with pathogens.Notably,DAA-mediated dynamic crosslinking not only maintains the overall integrity of the hydrogels but also controls the release timing of the probiotics.Thus,it is expected that encapsulated substances(probiotics,proteins,etc.)can be delivered to specific sites of the intestinal tract by means of DPH,by con-trolling the dynamic covalent crosslinking.
Wen-Can Huang、Wenjie Wang、Wei Wang、Yanan Hao、Changhu Xue、Xiangzhao Mao
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State Key Laboratory of Marine Food Processing and Safety Control,College of Food Science and Engineering,Ocean University of China,Qingdao 266404,China
Qingdao Key Laboratory of Food Biotechnology,Qjngdao 266404,China
Key Laboratory of Biological Processing of Aquatic Products,China National Light Industry,Qingdao 266404,China
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China
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National Natural Science Foundation of ChinaChina Agriculture Research System of the MOF and MARANatural Science Foundation of Shandong ProvinceTaishan Scholar Project of Shandong ProvinceFundamental Research Funds for the Central Universities
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