A Novel Light-Responsive Gene BcCfaS Regulates the Photomorphogenesis and Virulence of Botrytis cinerea via Lipid Metabolism

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外文摘要：Light is a fundamental environmental factor for living organisms on earth-not only as a primary energy source but also as an informational signal.In fungi,light can be used as an indicator for both time and space to control important physiological and morphological responses.Botrytis cinerea(B.cinerea)is a devastating phytopathogenic fungus that exploits light cues to optimize virulence and the balance between conidiation and sclerotia development,thereby improving its dispersal and survival in ecosys-tems.However,the components and mechanisms underlying these processes remain obscure.Here,we identify a novel light-signaling component in B.cinerea,BcCfaS,which encodes a putative cyclopropane fatty-acyl-phospholipid synthase.BcCfaS is strongly induced by light at the transcriptional level and plays a crucial role in regulating photomorphogenesis.Deletion of BcCfaS results in reduced vegetative growth,altered colony morphology,impaired sclerotial development,and enhanced conidiation in a light-dependent manner.Moreover,the mutant exhibits serious defects in stress response and virulence on the host.Based on a lipidomics analysis,a number of previously unknown fungal lipids and many BcCfaS-regulated lipids are identified in B.cinerea,including several novel phospholipids and fatty acids.Importantly,we find that BcCfaS controls conidiation and sclerotial development by positively regulating methyl jasmonate(MeJA)synthesis to activate the transcription of light-signaling components,revealing for the first time the metabolic base of photomorphogenesis in fungi.Thus,we propose that BcCfaS serves as an integration node for light and lipid metabolism,thereby providing a regulatory mechanism by which fungi adapt their development to a changing light environment.These new findings provide an important target for antifungal design to prevent and control fungal disease.

外文关键词：

LightBotrytis cinereaCyclopropane fatty-acyl-phospholipidsynthaseLipid metabolismPhotomorphogenesisVirulence

作者：

Guangjin Li、Zhanquan Zhang、Yong Chen、Tong Chen、Boqiang Li、Shiping Tian

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作者单位：

Key Laboratory of Plant Resources,The Innovative Academy of Seed Design,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China

University of Chinese Academy of Sciences,Beijing 100049,China

基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development(R&D)Program of ChinaNational Key Research and Development(R&D)Program of China

项目编号：

31930086321726422016YFD04009022021YFD2100505

出版年：

2024

DOI：

10.1016/j.eng.2023.08.015

工程(英文)

CSTPCDEI

ISSN：2095-8099

年,卷(期)：2024.35(4)