Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms

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外文摘要：This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil processes.The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate production.Compared with anaerobic sludge and pit mud,the native soil CE system exhib-ited higher selectivity(60.53％),specificity(82.32％),carbon distribution(60.00％),electron transfer effi-ciency(165.00％),and conductivity(0.59 ms·cm-1).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate production.Moreover,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO2 utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE process.Overall,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental ben-efits through soil consortia exploration.

外文关键词：

SoilChain elongationn-caproateReverse beta-oxidationFatty acid biosynthesisMetagenomics

作者：

Lin Deng、Yang Lv、Tian Lan、Qing-Lian Wu、Wei-Tong Ren、Hua-Zhe Wang、Bing-Jie Ni、Wan-Qian Guo

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作者单位：

State Key Laboratory of Urban Water Resource and the Environment,Harbin Institute of Technology,Harbin 150090,China

Centre for Technology in Water and Wastewater & School of Civil and Environmental Engineering,University of Technology Sydney,Sydney,NSW 2007,Australia

基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaOpen Project of State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of TechnologyFundamental Research Funds for the Central Universities

项目编号：

5200013251978201HC202241

出版年：

2024

DOI：

10.1016/j.eng.2023.10.017

工程(英文)

CSTPCDEI

ISSN：2095-8099

年,卷(期)：2024.39(8)