内置三维结构的壁载纤维素酶微反应器能够高效转化纤维二糖制备葡萄糖，作为后续发酵制取生物燃料的原料。构建了内置长方体结构的壁载纤维素酶微反应器，在纤维二糖浓度50 g·L-1，流量0。2 min mL-1的条件下，10 min的葡萄糖得率高达93。0％。基于上述过程建立了耦合流体流动、物质传递、酶催化反应的数学模型，探究了结构形状、间距、大小等参数对传递及转化的影响规律。在底物浓度20 g·L-1、流量1。0 mL·min-1的条件下，三棱柱结构的设置使得微反应器内葡萄糖表面生产率及葡萄糖得率分别提升了 39。9％及143。0％。而长方体结构能够使微反应器在单位压降下获得最高的葡萄糖产量，其性能参数最高为6。83 g·h-1·Pa-1，相较于表面无结构的微反应器提升了 51。4％，有利于实际应用。
Transport and Conversion Characteristics in a Cellulase-Loaded Microreactor With Built-in Three-Dimensional Structures
The cellulase-loaded microreactor with built-in three-dimensional(3D)structures is ca-pable of efficiently converting cellobiose to prepare glucose as the feedstock for subsequent fermenta-tion to produce biofuels.The glucose yield in the microreactor with built-in rectangular structures reached 93.0％in 10 min at a substrate concentration of 50 g·L-1 and a flow rate of 0.2 min·mL-1.A mathematical model coupling with fluid flow,mass transfer,and enzymatic reaction was constructed based on the above process and the effect of different structure shapes,spacings,sizes and other parameters on mass transfer and conversion was explored.At a substrate concentration of 20 g·L-1 and a flow rate of 1.0 mL·min-1,the setup of triangular prism structure in the microreactor led to an increase in glucose surface productivity and glucose yield by 39.9％and 143.0％.Meanwhile,the cuboid structure enables the microreactor to obtain the highest glucose yield per unit pressure drop,with a performance parameter of 6.83 g·h-1·Pa-1,which is 51.4％higher than that in the microreactor with no surface structures,which is conducive to practical applications.
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