Design of automatic platelet immunofluorescence staining control system
Blood biopsy technology based on visualized platelet ultrastructure has important applications in the monitoring and diagnosis field of acute ischemic stroke and other diseases.However,current platelet immunofluorescence staining heavily relies on manual operation,with heavy workload,low efficiency and poor consistency,and it is difficult to cope with the increasing demand for blood sample monitoring.In order to solve the shortage of manual staining and make up for the blank of automatic platelet immunofluorescence staining equipment in China,an automatic platelet immunofluorescence staining control system was invented,which mainly included power module,main control module,sensor feedback module,motion execution module and upper computer software.The control system could realize the accurate control of the immunofluorescence staining operations with ten sample fluxes,including drilling,sealing,primary antibody labeling,primary antibody rinsing,secondary antibody labeling,secondary antibody rinsing,PFA(polyformaldehyde fixative)fixation and rinsing.The test results showed that the designed control system could control the repeated positioning accuracy of the X,Y and Z axes within±0.1 mm,the accuracy of adding trace reagents reached 1 μL,the accuracy of adding a large amount of reagents reached 1 mL,and the failure rate of multiple experiments was less than 1%.It could reduce the cost of platelet immunofluorescence staining while obtaining a clear and determinable staining result comparable to manual staining.The automatic platelet immunofluorescence staining control system has the advantages of high stability,high accuracy and high efficiency,and the obtained fluorescence staining results of platelet ultrastructure have high clarity under super-resolution microscope,which can provide an efficient experimental tool for the detection of platelet ultrastructure based on super-resolution technology,and has important engineering significance.
platelet ultrastructure detectionautomatic immunofluorescence stainingcontrol system
万方数据
维普
