Surface and in vitro osteogenic activity of osteogenic growth peptide-decorated polyetheretherketone
In order to improve the osteogenic activity of polyetheretherketone(PEEK),polydopamine(PDA)coating was first formed on its surface by oxidative self-polymerization of dopamine(DA)under alkaline conditions,and then osteogenic growth peptide(OGP)with osteogenic activity was chemically bonded to its surface by PDA.The surfaces of PEEK before and after modifi-cation were characterized by X-ray photoelectron spectroscopy(XPS),scanning electron microscopy(SEM)and water contact angle test.Meanwhile,MC3T3-E1 osteoblasts were used to evaluate the in vitro osteoblast compatibility and osteogenic activity of surface unmodified and modified PEEK.The XPS results show that PDA coating is successfully formed on PEEK surface,and OGP is chemically bonded to PEEK surface by PDA.The results of SEM and water contact angle test show that the surface morphology of PEEK after PDA and OGP modification do not change significantly,but the hydrophilicity of PEEK surface after PDA and OGP modification gradually increases.The evaluation results of osteoblasts MC3T3-E1 in vitro show that both PDA and OGP modified PEEK surfaces improve the adhesion,spreading,proliferation and osteogenic differentiation activity of osteoblasts MC3T3-E1 compared with unmodified PEEK.Among them,OGP modified PEEK surface shows the best of osteoblast adhesion,spreading,proliferation and osteogenic differentiation activity.The above results indicate that PEEK surface modified with OGP can effectively improve its osteoblast compatibility and osteogenic activity,and enhancing its potential in clinical fields such as orthopedics.
NETL
NSTL
万方数据
