Study on the Application of Ethyl Acetate in Refining White Birch Wood Vinegar
Using white birch wood vinegar as a raw material,a method of static filtration ethyl acetate extraction rotary membrane evaporation was used to refine it.The active components were analyzed by gas chromatography-mass spectrometry(GC-MS)and their effects on the germination of alfalfa(Medicago sativa)seeds were studied.GC-MS analysis identified 71 volatile components,mainly ketones,phenols,organic acids,and other substances,among which the relatively high content of compounds is phenyl carbamate(9.23％)γ-sitosterol(7.46％),3-methyl-phenol(6.10％),guaiacol(5.09％),etc;The results of seed germination experiments with refined white birch wood vinegar diluted at 100,500,1000,1500,and 2000 times showed that refined white birch wood vinegar diluted at different times had a significant effect on the germination of alfalfa seeds.When diluted at 1000 times,the effect was the strongest,with the highest germination rate of 92.00％,which was 30.67％higher than the control group,with a significant difference(P＜0.05).
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