Determination of Constituents Potentilla Fragarioides
This study analyzed Chemical constituents of Potentilla fragarioides extract,and HPLC determination method were established for three main chemical constituents((E)-caffeoylisocitric acid,(+)-catechin,quercetin-3-o-β-D-glucuronide)in the Potentilla fragarioides L.The result showed that a good linear relationship(r≥0.999),and the average recoveries(n=6)were 103.15％～103.90％(RSD＜3％,n=6).The contents of(E)-caffeoylisocitric acid,(+)-catechin,quercetin-3-o-β-D-glucuronide were 1.67 mg/g,0.20 mg/g and 0.95 mg/g,respectively.This method was simple,repeatable,accurate and reliable,and could be used for the quality control and evaluation,provide scientific basis for the research of pharmacodynamic substances of potentilla fragarioides L.It also promote the establishment of quality standard,quality control and further development and utilization of potentilla fragarioides.
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