Experimental Study on Inhibitory Effect of Sporosarcina Pasteurii on Oxidative Acid Production from Pyrite and the Fixation Effect of Heavy Metals
A large number of tailings and waste rocks generated from mining and beneficiation activities in mines are mostly deposited in the open air.The untreated acid mine drainage(AMD)generated from the oxidation of sulfide-containing minerals with oxygen,water and microorganisms will cause severe environmental pollution and even threaten people's life.It is considered to be the main environmental problem facing the global mining industry.At present,the commonly used treatment method is terminal treatment,which treats the symptoms rather than the root cause,so it is particularly important to carry out effective source control.In order to explore the control of AMD production from the source,pyrite was used as the research object,Sporosarcina pasteurii was selected and H2O2 was added as an oxidant to simulate the oxidation environment for a 10-day real-time monitoring experiment and heavy metal pollution solution remediation experiment,and the inhibitory effect and heavy metal fixation effect of bacteria on pyrite oxidation acid production behavior and heavy metal fixation effect were investigated from pH,Eh,SO42-and the concentration of heavy metal ions.The results showed that under the carbonate buffer provided by bacteria,the pH of the system was increased to neutral to alkaline,and Eh was maintained in a low-potential reducing environment,while the concentration of SO42-was reduced.The removal rate of heavy metal ions by bacteria in response to its induced coprecipitation and cell wall adsorption mechanism is above 80％.The results show that it is effective in inhibiting pyrite oxidation to produce acid and has good heavy metal fixation effect.
acid mine drainagepyriteSporosarcina pasteuriiheavy metal
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