A Methodological Study on the Determination of Characteristic Components of Nepetalactone in the Volatile Oil of Nepeta angustifolia C.Y.Wu by HPLC
Objective:To establish a method for the determination of E,Z-nepetalactone in volatile oil of Nepeta angustifolia C.Y.Wu by HPLC.Methods:Octadecylsilane bonded silica gel was used as filler(column:Supersil ODS 250 mm×4.6 mm,5 μm),acetonitrile-water(40∶60)as HPLC mobile phase,the flow rate was 1 mL/min,the column temperature was 30 ℃,and the detection wavelength was 216nm.Results:The linear relationship equation was y=1E+07x+287501,R2=0.9991,and the peak area in the range of 0.0098～2.4405 mg/mL had a good linear relationship with the injection concentration.The precision and repeatability were good,the RSD values of peak area were 0.66％and 0.32％,respectively,the average recovery rate was 101.67％,and the sample solution was stable within 24 hours.Conclusion:It was confirmed for the first time that the volatile oil of Nepeta angustifolia C.Y.Wu contains E,Z-nepetalactone.The established high-performance liquid chromatography method is simple,accurate,rapid,reproducible and specific,and can be applied to the determination of the characteristic components of E,Z-nepetalactone.
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