HPLC法测定藏荆芥挥发油中荆芥内酯特征成分含量的方法学研究
A Methodological Study on the Determination of Characteristic Components of Nepetalactone in the Volatile Oil of Nepeta angustifolia C.Y.Wu by HPLC
蔡鸿飞 1张琴 2毛禹康 2韩亚明 2杨阳 2张圳 2李康强 1许文东1
作者信息
- 1. 广州白云山汉方现代药业有限公司 中药制药过程技术与新药创制国家工程研究中心,广东广州 510240;广药汉方(珠海横琴)药业有限公司,广东珠海 519000
- 2. 广州白云山汉方现代药业有限公司 中药制药过程技术与新药创制国家工程研究中心,广东广州 510240
- 折叠
摘要
目的:建立HPLC法测定藏荆芥挥发油中E,Z-荆芥内酯特征成分含量的方法.方法:以十八烷基硅烷键合硅胶为填充剂(色谱柱:Supersil ODS 250mm×4.6mm,5μm),以乙腈-水(40∶60)为HPLC的流动相,流速为1 mL/min,色谱柱的柱温为30 ℃,检测波长为216nm.结果:线性关系方程为y=1E+07x+287501,R2=0.9991,在0.0098～2.4405 mg/mL范围内的峰面积与进样浓度线性关系良好.精密度和重复性良好,峰面积RSD分别为0.66％、0.32％,加样回收率平均值为101.67％,24h内样品溶液稳定.结论:首次确认藏荆芥挥发油中含有E,Z-荆芥内酯,所建立的高效液相色谱法方法简单、准确、快速,重复性强,专属性好,可应用于藏荆芥E,Z-荆芥内酯特征成分的含量测定.
Abstract
Objective:To establish a method for the determination of E,Z-nepetalactone in volatile oil of Nepeta angustifolia C.Y.Wu by HPLC.Methods:Octadecylsilane bonded silica gel was used as filler(column:Supersil ODS 250 mm×4.6 mm,5 μm),acetonitrile-water(40∶60)as HPLC mobile phase,the flow rate was 1 mL/min,the column temperature was 30 ℃,and the detection wavelength was 216nm.Results:The linear relationship equation was y=1E+07x+287501,R2=0.9991,and the peak area in the range of 0.0098～2.4405 mg/mL had a good linear relationship with the injection concentration.The precision and repeatability were good,the RSD values of peak area were 0.66％and 0.32％,respectively,the average recovery rate was 101.67％,and the sample solution was stable within 24 hours.Conclusion:It was confirmed for the first time that the volatile oil of Nepeta angustifolia C.Y.Wu contains E,Z-nepetalactone.The established high-performance liquid chromatography method is simple,accurate,rapid,reproducible and specific,and can be applied to the determination of the characteristic components of E,Z-nepetalactone.
关键词
藏荆芥/E,Z-荆芥内酯/含量测定/高效液相色谱/方法学Key words
Nepeta angustifolia C.Y.Wu/E,Z-nepetalactone/content determination/high performance liquid chromatography/methodology引用本文复制引用
基金项目
广东省重点领域研发计划(2020B1111120002)
广州市科技计划项目(202002020009)
广东省科技计划项目广东省药用脂质重点实验室(2023B1212070030)
出版年
2024
NETL
NSTL
万方数据