摘要
活性氧物种(ROS)如过氧化氢(H2O2)、次氯酸(HC1O)、过氧化亚硝基阴离子(ONOO-)、超氧阴离子(O-2)、羟基自由基(HO·)、单线态氧(1O2)等,它们是广泛存在于生物体内具有高反应活性的含氧物质.活性氧物种在生物体内的时空分布在癌症、关节炎、阿尔兹海默症等疾病领域的研究得到空前的关注.而比例型活性氧荧光探针以其高灵敏度、准确度、多通道原位成像的优势为这一领域提供了有力的工具.比例型荧光探针的设计需要借助多种光物理特性,本文就光物理特性分类按照分子内电荷转移(ICT)、荧光共振能量转移(FRET)、激发态分子内质子转移(ESIPT)、跨键能量转移(TBET)等介绍活性氧比例型荧光探针的最新进展.
Abstract
Reactive oxygen species(ROS)such as hydrogen peroxide(H2O2),hypochlorous acid(HC1O),nitroso-peroxide anion(ONOO),superoxide anion(O2),hydroxyl radical(HO·),singlet oxygen(1O2),etc.,which are widely present in organisms with high reactive oxygen activity.The spatial and temporal distribution of reactive oxygen species in living organisms has received unprecedented attention in the field of diseases such as cancer,arthritis,and Alzheimer's disease.Ratiometric fluorescent probes for ROS provide powerful tools for this field with their high sensitivity,accuracy and multi-channel in situ imaging.The design of ratiometric fluorescent probes requires a variety of photophysical properties,and this paper introduces the latest progress of ratiometric fluorescent probes for ROS according to intramolecular charge transfer(ICT),fluorescence resonance energy transfer(FRET),excited state intramolecular proton transfer(ESIPT),and Through-bond energy transfer(TBET).
基金项目
新疆维吾尔自治区自然科学基金(2021D01A91)
NETL
NSTL
万方数据