Study on the Mechanism of Liver Injury Induced by Polygonum Multiflorum Based on Network Pharmacology
Objective:To screen the target of liver damage by chemical constituents of Polygon um multiflorum by network pharmacology techniques and methods,and to construct a component-target-hepatic injury network map to explore the mechanism of liver damage caused by Polygonum multiflorum.Methods:The chemical constituents of Polygonum multiflorum were retrieved from the TCMID,TCMGenDIT database and the published literature,The TCMP and BATMAN-TCM databases were used to screen and obtain the corresponding targets.The results were searched by DrugBank,DisGeNET and GeneCards.Targets of liver injury,integrated analysis of intersection targets to establish STRING protein interactions,construction of component-target-hepatic injury visualization network using Cytoscape 3.7.1 software;analysis of target gene function(GO)by DAVID database and based on Kyoto Gene and Genomic Encyclopedia(KEGG)pathway enrichment analysis.Results:A total of 38 chemical components of Polygonum multiflorum,307 component targets,6140 liver injury targets,and 252 target of intersection of components and liver injury were collected,838 GO functional enrichment analysis,and KEGG pathway enrichment analysis was 111.It is predicted that the pathways involved in liver injury mainly include cAMP signaling pathway,MAPK signaling pathway,nonalcoholic fatty liver disease(NAFLD),HIF-1 signaling pathway,drug metabolism-cytochrome P450,TNF signaling pathway,PI3K-Akt Signaling pathway,NOD-like receptor signaling pathway,Toll-like receptor signaling pathway,Wnt signaling pathway.Conclusion:The characteristics of multi-component,multi-target and multi-channel interaction of Polygonum multiflorum were further explained,which provided new ideas and new directions for the study of the mechanism of liver injury induced by Polygonum multiflorum.
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