Based on Network Pharmacology,the Mechanism of Chest Relief Formula in the Treatment of Coronary Heart Disease was Explored
Objective:To study the molecular mechanism of Chest Relaxation in the treatment of coronary heart disease(CHD).Methods:TCMSP was used to obtain the chemical components of safflower,Chuanxiong and Panax notoginseng in Shu Chest Fang,and the active ingredients and coronary heart disease targets of Shu Chest Fang were predicted by GeneCards and OMIM databases;The component-target PPI network diagram was plotted using the STRING database;The"component-target-disease"network diagram was drawn by Cytoscape software,and the micro-bioinformatics platform was used to perform GO and KEGG enrichment analysis for the obtained target and compound molecular information;PDB,Pubchem database,and AutoDockTools software were used to molecularly dock the core compounds of Chest Relaxation Fang and the core targets of CHD.Results:497 chemical components and 8408 CHD-related targets were screened;After 353 intersecting targets were obtained by PPI network analysis,30 core targets were found according to Degree,and the main core targets included SRC,STAT3,PIK3R1,etc.GO functional enrichment analysis showed that 112 items related to biological processes,102 items related to molecular function,and 63 items related to cellular components were obtained(P＜0.05);KEGG pathway enrichment analysis showed 164 pathways including P13K-Akt and MAPK(P＜0.05),The molecular docking results showed that lignans and SRC,6-hydroxyflavones and STAT3,and ponylase ketones had high docking activity with PIK3R1.Conclusion:A variety of active ingredients such as lignans,6-hydroxyflavonoids,and syrylase ketones may treat CHD by inhibiting the gene expression of SRC,STAT3,and PIK3R1,thereby regulating PI3K-AKT,MAPK and other signaling pathways.
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