Chemical Compositions and Antioxidant Activity of Mushroom Oil of Calvatia Candida(Schw.)Fr.
To analysis the chemical compositions of the mushroom oil of Calvatia candida(Schw.)Fr.and to evaluated its DPPH radical scavenging activity,the crude fat was obtained from Calvatia candida(Schw.)Fr.using the method of continuous reflux extraction with diethyl ether,and the crude fat was further purified by silica gel short column to obtain the mushroom oil.The chemical composition of the mushroom oil was analyzed by gas chromatography-mass spectrometry(GC-MS),and the antioxidant activity of the mushroom oil was evaluated by the in vitro DPPH radical scavenging assay.The results showed that the crude fat yield was 9.49%and the mushroom oil yield was 0.28%.By GC-MS analysis,49 compounds were identified in the mushroom oil of Calvatia candida(Schw.)Fr.,the relative content of which was 73.562%of the total mushroom oil.The main characteristic chemical components were 6 fatty acids and their esteroid derivatives,among them,there were 4 unsaturated fatty acids and their esters,methyl linoleate,methyl oleate,cis-13-octadecenoic acid,and ethyl linoleate,with a total relative content of 51.06%,and there were 2 saturated fatty acids and their esters,namely ethyl palmitate and methyl palmitate,with a total relative content of 12.55%.The results of free radical scavenging assay showed that the mushroom oil has effective DPPH radical scavenging activity,with half scavenging concentration(EC50)was 26.1362±0.6435 mg/mL.
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