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高效液相色谱-紫外检测法测定化妆品中咖啡因的含量

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建立了高效液相色谱法测定化妆品中咖啡因的含量.试样经甲醇提取稀释后或正己烷分散再用甲醇水溶液反萃提取净化后,采用Waters Atlantis T3色谱柱(4.6 mm×150 mm,5 μm)分离,以甲醇和水为流动相梯度洗脱,流速为1.0 mL/min,检测波长为 273 nm,柱温为40℃.结果表明,咖啡因在 0.2~10 mg/kg的质量浓度范围内呈现良好线性关系,相关系数为 0.9999,七种基质三个浓度水平的平均回收率在105.5%~110%之间,RSD在 0.7%~4.5%之间,方法的定量限为10 mg/kg.建立的方法具有提取效率高、分析时间短、测试结果准确可靠、重复性好等优点,可适用于化妆品中咖啡因的含量测定.
Determination of Caffeine in Cosmetics by High Performance Liquid Chromatography-Ultraviolet Detection
A method was developed for the determination of caffeine in cosmetics products by high performance liquid chromatography(HPLC).Samples were extracted with methanol and then diluted well or dissolved in hexane and then reverse extracted by methanol and water.The samples were separated on a Waters Atlantis T3(4.6 mm×150 mm,5 μm)HPLC column with the mobile phases of methanol and water.The flow rate was 1.0 mL/min under gradient elution,the detection wavelength was 273 nm and the column temperate was 40℃.The results show that the calibration curves had good linearity in the range of 0.2 mg/kg to 10 mg/kg,with correlation coefficients greater than 0.9999.The average recoveries at the three spiked levels of seven matrixes were in the range of 105.5%to 110%and the relative standard deviations(RSDs)are in the range of 0.7%to 4.5%.The limit of quantification was 10 mg/kg.With the advantages of convenience,sensitivity,good recoveries,efficient and accurate,the established method can be used for the detection of caffeine in cosmetics products.

high performance liquid chromatographyultraviolet detectioncaffeinecosmeticsmethodology

陈思敏、吴映璇、庄燕君、邵琳智

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广州海关技术中心,广东 广州 510623

高效液相色谱法 紫外检测 咖啡因 化妆品 方法学

关级项目关级项目

2020GZCK-0082020GZCK-023

2024

广东化工
广东省石油化工研究院

广东化工

影响因子:0.288
ISSN:1007-1865
年,卷(期):2024.51(3)
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