Determination of 20 Illegally Added Anti-infective Drugs in Acne Cosmetics by HPLC-Q-TOP-MS
Objective:To establish a method for detecting 20 illegal anti-infective drugs in acne cosmetics by HPLC-Q-TOP-MS.Methods:An Agilent EC-C18 column(3.0×150 mm,2.7 μm)was adopted at the flow rate of 0.3 mL/min with the mobile phase was acetonitrile-0.1％glacial acetic acid solution,and the gradient eluted;The column temperature is 35℃;Positive ion mode electrospray ionization,based on the retention time,precursor ion,fragment ion accurate mass and other data extracted by MassHunter,a screening database for illegally added anti-infective drugs was constructed in PCDL.Results:Chloramphenicol and spironolactone showed a good linear relationship(r>0.99)in the range of mass concentration of 100～5000 ng/mL,while the remaining 18 anti-infective drugs showed a good linear relationship in the range of mass concentration of 50～5000 ng/mL.The detection limit was 0.01～0.20 μg/g,the quantification limit was 0.05～0.80 μg/g,the recovery rate was 83.7％～108.8％,and the relative standard deviation(RSD)was 1.2％～8.1％.Conclusion:This method is simple,accurate and reliable,and is suitable for the rapid screening of 20 anti-infective drugs in acne cosmetics,which provides strong technical support for the safety supervision of acne cosmetics.
NETL
NSTL
万方数据
