Study on Fingerprint and Chemical Component Content Determination of Xihuangcao Broken Wall Decoction Pieces
Objective:To establish a method for the determination of the content of cut-wall cut-pieces by HPLC fingerprint,and to provide a reference for the quality control of cut-wall cut-pieces.Method:A HPLC method was established for the determination of CAFFEIC ACID and rosmarinic acid in cut crude drug of Herba Secalinis.The chromatographic column was Platisil C18(5 μm,4.6×250 mm)and the mobile phase was acetonitrile-0.1%phosphoric acid,the flow rate was 1.0 mL/min,the column temperature was 35℃,and the detection wavelength was 327 nm.The chromatographic data were analyzed by the similarity evaluation system of chromatographic fingerprints of Chinese Materia Medica of the State Pharmacopoeia Commission,and the standard fingerprints were generated.Result:There were 7 common peaks in the 10 batches of cut-off pieces,the similarity was 0.867~0.983,and 2 chromatographic peaks were identified.There was a good linear relationship between caffeic acid and rosmarinic acid in the range of 0.0354~0.1239 mg/mL(R2=0.9999)and 0.0866~0.3030 mg/mL(R2=0.9999).The RSD of repeatability test,stability test and precision test were all<2%.The recoveries of caffeic acid,rosmarinic acid and rosmarinic acid were 91.4%~94.6%,1.3%;93.6%~97.5% and 1.6%,respectively.Conclusion:The method is accurate,reliable and reproducible,and provides scientific basis for the quality control of Saussurea Chinense.