首页|基于光学显微技术的淀粉粒计量方法及其在中药三七中的应用

基于光学显微技术的淀粉粒计量方法及其在中药三七中的应用

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目的:建立基于光学显微技术的淀粉粒计量方法,并应用于中药三七中淀粉粒的计量.方法:基于光学显微技术的原理,利用淀粉遇碘液变蓝紫色的性质,采用稀碘液对淀粉粒染色;利用Image J软件对染色的淀粉粒进行自动计量.以中药三七粉末样品为测量材料,对稀碘液浓度、样品浓度、显微测量区域进行考查.结果:以0.002 mol/L浓度的稀碘液、15 mg/mL的样品浓度、显微测量9 个区域为最佳实验条件,对样品中的淀粉粒面积占比进行测量.此方法的精密度、重复性、重现性的RSD均小于10.5%,表明该方法稳定、可靠.采用该方法测定三七中淀粉粒,结果表明不同规格等级三七中淀粉粒的面积占比差异均有统计学意义,淀粉粒变化规律为:40 头>60头>120头>无数头>筋条>绒根≈剪口.结论:本研究建立基于光学显微技术的淀粉粒计量方法,在不同规格等级三七中得到很好的应用,为植物样品中淀粉粒显微计量提供了有效方法.
A Metering Method of Starch Granules Based on Optical Microscopy and its Application to Panax Notoginseng
Objective:To establish a metering method of starch granules based on optical microscopy and to applicate the method to Panax notoginseng.Methods:The starch granules were stained with dilute iodine solution and observed by an optical microscopy.Image J software was used for the auto measurement of the area percentage of starch granules in the slide.The concentration of dilute iodine solution,the concentration of sample,and the microscopic measurement zone were validated using the powder of Panax notoginseng as a sample.Results:Starch granules was well stained in a dilute iodine solution with a concentration of 0.002 mol/L.The density of starch granules in a sample with a concentration of 15 mg/mL was appropriate for measurement.The result was reliable when the microscopic measurement zones were nine.The RSD of precision,repeatability,reproducibility of the starch granules metering method were all<10.5%,indicating the method was stable and reliable.The starch granules in Panax notoginseng powder was metered by this method.The results indicated that there was significant difference of the starch granules of number different specifications and grades of Panax notoginseng.The area percentage of starch granules was in an order of 40-Tou>60-Tou>120-Tou>Coutless-Tou>Jintiao>Ronggen≈Jiankou.Conclusion:The metering method of starch granules established by optical microscopy is simple,fast and reliable.The method has been well applied to Panax notoginseng,and will effective support for the metering of starch granules in the plant materials.

starch granulesoptical microscopymetering methodpanax notoginsengspecifications and gradesImage J software

杨迪、杨杰、鲁瑶、邸学、李娜、王添敏

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辽宁中医药大学,辽宁 大连 116600

淀粉粒 光学显微技术 计量方法 三七 规格等级 ImageJ软件

国家自然科学基金辽宁省自然科学基金沈阳市中青年科技创新人才支持计划

817036582019-MS-223RC190083

2024

广东化工
广东省石油化工研究院

广东化工

影响因子:0.288
ISSN:1007-1865
年,卷(期):2024.51(5)
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