四环素长期暴露对脱氮系统性能及微生物群落结构的影响

Effects of Long-term Tetracycline Exposure on the Performance of Denitrification Systems and Microbial Communities

孙雪宁 ¹孙洪伟 ¹孙茜¹

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作者信息

1. 烟台大学,山东烟台 264005
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摘要

本实验通过构建序批式反应器(SBR),研究四环素浓度为0～35 mg/L的废水对硝化脱氮系统性能及微生物群落结构的影响.实验结果表明,四环素浓度≤20 mg/L时,硝化菌的活性随着暴露周期的增加而逐渐恢复.在四环素浓度为35 mg/L时,硝化菌失去活性,硝化系统崩溃.通过微生物群落结构分析可知,四环素的存在使Proteobacteria菌门产生一定的耐药性.随着四化素的浓度增加,对Ferribacterium和Nitrospira菌属的抑制程度越来越强,对Zoogloea和Pseudoxanthomonas菌属的抑制作用越来越弱.另外,四环素浓度为5 mg/L时利于Thauera菌属的生长.四环素浓度为35 mg/L时Nitrosomonas菌属全部失活.

Abstract

In this experiment,a sequencing batch reactor(SBR)was constructed to investigate the effects of wastewater with tetracycline concentrations of 0～35 mg/L on the performance of the nitrification and denitrification system and the structure of the microbial community.The experimental results showed that the activity of nitrifying bacteria at tetracycline concentrations≤ 20 mg/L gradually recovered with the increase of the exposure cycle.At a tetracycline concentration of 35 mg/L,the nitrifying bacteria lost their activity and the nitrification system collapsed.Analysis of the microbial community structure showed that the presence of tetracycline caused the Proteobacteria phylum to develop some resistance.With increasing tetracycline concentration,the degree of inhibition of Ferribacterium and Nitrospira spp.became stronger and the inhibition of Zoogloea and Pseudoxanthomonas spp.became weaker.In addition,a tetracycline concentration of 5 mg/L favoured the growth of Thauera spp.Nitrosomonas spp.were inactivated at a tetracycline concentration of 35 mg/L.

关键词

四环素/硝化系统/活性污泥/生物脱氮/长期暴露

Key words

achromycin/nitrification system/activated sludge/biological removal of nitrogen/long-term exposure

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基金项目

山东省自然科学基金面上项目(ZR2020ME226)

出版年

2024

广东化工

广东省石油化工研究院

广东化工

影响因子：0.288

ISSN：1007-1865

参考文献量8

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