草石蚕多糖的制备及其对高糖诱导大鼠肾小球系膜细胞的影响
Preparation of Polysaccharides from Stachys Sieboldii and their Effect on High Glucose-induced Rat Glomerular Mesangial Cells
郭海慧 1殷瑞 1王福生1
作者信息
- 1. 大理大学 药学院,云南 大理 671000
- 折叠
摘要
为研究草石蚕多糖对高糖诱导大鼠肾小球系膜细胞(HBZY-1)的影响.草石蚕水提液经脱蛋白、AB-8 大孔树脂柱层析分离,得草石蚕粗多糖并进行分级醇沉分离得到30%醇沉组分(SSMP-30%)、60%醇沉组分(SSMP-60%)和90%醇沉组分(SSMP-90%),其中SSMP-90%的含量最高.以高糖诱导HBZY-1 为细胞模型对醇沉组分进行体外活性测试.结果表明,不同醇沉组分对模型细胞均无毒性,与高糖对照组比较,SSMP-30%和SSMP-90%组分对高糖诱导HBZY-1 的增殖具有显著抑制作用(P＜0.01),且呈浓度依赖性,其中SSMP-90%的活性最强.提示其对高糖诱导大鼠肾小球系膜细胞具有保护作用.
Abstract
In order to study the effect of the polysaccharides from S.sieboldii on high glucose-induced glomerular mesangial cells(HBZY-1)in rats.The aqueous extract of S.sieboldii was deproteinised and separated by AB-8 macroporous resin column chromatography to obtain the crude polysaccharides of S.sieboldii,and then subjected to graded alcoholic precipitation to obtain the 30%alcoholic precipitation fraction(SSMP-30%),60%alcoholic precipitation fraction(SSMP-60%)and 90%alcoholic precipitation fraction(SSMP-90%),in which SSMP-90%had the highest content.The in vitro activity of the alcohol precipitated fractions was tested using high glucose-induced HBZY-1 as a cell model.The results showed that the different alcohol precipitation fractions were non-toxic to the model cells,and compared with the high glucose control group,the SSMP-30%and SSMP-90%fractions had a significant inhibitory effect on the proliferation of high glucose-induced HBZY-1(P＜0.01)in a concentration-dependent manner,in which SSMP-90%had the strongest activity.It was suggested that polysaccharides from S.sieboldii had a protective effect on high glucose-induced glomerular mesangial cells in rats.
关键词
草石蚕/分级醇沉/肾小球系膜细胞/高糖诱导/糖尿病肾病Key words
Stachys sieboldii polysaccharides/graded alcohol precipitation/glomerular mesangial cells/high glucose-induced/diabetic nephropathy引用本文复制引用
基金项目
云南特色植物提取实验室开放研究项目(YKKF2323005)
出版年
2024
NETL
NSTL
万方数据