芦丁分子印迹聚合物的制备及其应用
Preparation and Application of Rutin Molecularly Imprinted Polymer
侯星羽 1朱家莹 1沈景辉 1边新新 1丁立新1
作者信息
- 1. 佳木斯大学 药学院,黑龙江 佳木斯 154000
- 折叠
摘要
本文以MCM-48 为载体,为芦丁(RT)为模板分子,3-羧酸苯硼酸(CPBA)为功能单体制备芦丁分子印迹聚合物(RT-MIPs),使用紫外-可见分光光度计进行含量测定.优化制备条件,当CPBA为 50 mg、间苯二酚与甲醛用量摩尔比为 1∶2、pH为8.5时,RT-MIPs对目标分子RT的吸附能力最佳,吸附量为 14.80 mg/g.对实际样品(槐花、荞麦、玉米须、红枣)进行加标回收实验,回收率为95.43%～106.07%,该方法设计合理.表明RT-MIPs具有较好的实际应用前景,有望用于天然药物活性成分RT的快速选择性分离提取,为其分离和富集提供了一条新的途径.
Abstract
Using MCM-48 as the sacrificial carrier,rutin(RT)as the template molecule,and 3-carboxylic phenylboronic acid(CPBA)as the functional monomer,Rutin-based molecularly imprinted polymers(RT-MIPs)were prepared.The content was determined using a UV-Vis spectrophotometer;The preparation conditions were optimized.When CPBA is 50 mg,the molar ratio of resorcinol to formaldehyde is 1∶2,and pH is 8.5,the adsorption capacity of RT-IMPs to target molecule RT was the best,and the adsorption capacity was 14.80 mg/g.The actual samples(Sophora,buckwheat,corn,jujube)were marked recovery experiment,the recovery rate was 95.43%～106.07%,the design of this method was reasonable.This shows that RT-MIPs have promising practical applications and are expected to be used for the rapid and selective separation and extraction of the active ingredient RT in natural drugs,providing a new approach for their isolation and enrichment.
关键词
芦丁/硼亲和/分子印迹/聚合物/富集Key words
Rutin/boron affinity/molecularly imprinted/polymers/enrichment引用本文复制引用
出版年
2024
NETL
NSTL
万方数据