Optimization of Extraction Process of Zhuang Medicine Longlutong Granules by Orthogonal Experiment
Objective:To optimize the extraction process of Zhuang medicine Longlutong granules.Method:The content determination method of Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Rb1 was established by HPLC.The extraction amount,Notoginsenoside R1 content,Ginsenoside Rg1 content,Ginsenoside Rb1 content were used as evaluation indexes.The single factor method was used to investigate the amount of water added,extraction time,and extraction frequency,and the optimal extraction process for Zhuangyao Longlutong decoction was optimized through orthogonal test.Result:Taking the contents of Notoginsenoside R1,Ginsenoside Rg1,Ginsenoside Rb1 as the quality control standard of Longlutong granules,it was determined that the mass fractions of the three index components in Longlutong granules were Notoginsenoside R1 45.6～60.6 mg/g,Ginsenoside Rg1 159.3～234.4 mg/g,and Ginsenoside Rb1 143.8～246.0 mg/g;The optimal extraction process is to decoct with water three times,adding 12 times the amount of water for the first time,and adding 10 times the amount of water for the second and third times,each time for 1.5 hours.Conclusion:The optimized extraction process is reasonable and feasible,providing experimental basis for the production and further development of Zhuang meidicine Longlutong granules.
zhuang medicine Longlutong granulesorthogonal experimentextraction processextraction amountcontent determination
NETL
NSTL
万方数据
