Synthesis of Novel TTK Inhibitors N-10 and effects on Triple-negative Breast Cancer Cells
In this study,a novel TTK inhibitor N-10 was synthesized by chemical methods,and its inhibitory effect on TTK kinase,as well as its effect on the proliferation,colony formation,migration and invasion of triple-negative breast cancer cells was investigated.The target compound N-10 was synthesized from methyl 4-bromo-3-hydroxybenzoate by alkylation,hydrolysis,condensation and suzuki coupling reaction.The inhibitory effect of N-10 on TTK kinase was detected by enzyme experiments.The inhibitory effect of N-10 on the proliferation of triple negative breast cancer cells was detected by CellTiter-Glo assay.The effects of N-10 on migration and invasion ability of triple negative breast cancer cells were studied by cell scratch and Transwell invasion experiments.The experimental results showed that N-10 had a significant inhibitory effect on TTK kinase activity,and could effectively inhibit the growth and proliferation of triple negative breast cancer cells MDA-MB-231.The migration and invasion ability of triple negative breast cancer cells MDA-MB-231 was weakened,which provided scientific basis for further study of TTK inhibitor N-10.
drug synthesistriple negative breast cancerspindle assembly inspection pointTTK inhibitor
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