Optimization of Extraction Process of Total Flavonoids from Green Peel of Juglans sigillate Dode by Orthogonal Experiment
To optimize extraction technology of total flavonoids in the green peel of Juglans sigillate Dode.Total flavonoids from the green peel of Juglans sigillate Dode were extracted by reflux extraction method,taking the extraction rate of total flavonoids as index,the effects of extraction solvent,solid-liquid ratio,extraction time and extraction temperature on the extraction rate of total flavonoids were investigated.Based on the single factor experiment,the extraction process of total flavonoids was optimized by orthogonal test.The optimal extraction process was:50%ethanol solution as the extraction solvent,the ratio of solid to liquid was 1∶40,boiling water bath reflux extraction for 60 min.The content of total flavonoids was 9.81%.The content of total flavonoids from the green peel of Juglans sigillate Dode was high,and the Optimized extraction technology was stable with high extraction rate of total flavonoids.
Juglans sigillate Dodegreen peeltotal flavonoidsorthogonal testextraction process
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