高效液相色谱-串联质谱法分析人参配方颗粒中18种人参皂苷成分
Determination of 18 Ginsenosides in Ginseng Formulation Granules by High Performance Liquid Chromatography-Mass Spectrometry
庄玥 1黄艳婷 2韩毓之 2刘亚雄 2邱蕴绮2
作者信息
- 1. 广东工业大学,广东 广州 510006
- 2. 广东省药品检验所,广东 广州 510663
- 折叠
摘要
建立了高效液相色谱-串联质谱检测人参配方颗粒中18种人参皂苷定性定量的分析方法.样品经80%甲醇超声提取,采用Agilent Poroshell 120 EC-C18 色谱柱(2.1 mm×150 mm,2.7µm)分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱,电喷雾负离子模式,进行预设定多反应监测(sMRM)模式检测.结果显示,18种目标分析物在各自线性范围内线性良好,相关系数均大于0.9970.平均加标回收率为94.5%～105.2%,RSD为 1.6%～3.9%.采用本方法对 12份样品进行测定,发现4份样品中检测出拟人参皂苷成分.所建方法快速、可靠、灵敏,适用于人参配方颗粒中 18种人参皂苷的定性及定量分析.
Abstract
To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.Samples were extracted by 80%methanol with ultrasound extraction.The ginsenosides were separated on an Agilent Poroshell 120 EC-C18 column(2.1 mm×150 mm,2.7 μm)by gradient elution with 0.1%formic acid acetonitril and 0.1%formic acid water solution as mobile phase.A scheduled multiple reaction monitoring(sMRM)in negative ion mode was adopted in mass spectrometry acquisition.As a result,the linear correlation coefficients(r)of the 18 analytes in the linear ranges were more than 0.9970.The mean recoveries were all in the range of 94.5%～105.2%,and the relative standard deviations(RSD)were between 1.6%and 3.9%.Pse F11 was detected in 4 batches(12 batches in total)samples.The method was proved to be rapid,reliable and sensitive,which can be used to qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.
关键词
高效液相色谱-串联质谱/人参/人参皂苷/人参配方颗粒Key words
HPLC-MS/MS/ginseng/ginsenosides/ginseng formulation granules引用本文复制引用
基金项目
广东省医学科学技术研究项目(B2021409)
广东省中医药局科研项目(20221055)
出版年
2024
NETL
NSTL
万方数据