Determination of 18 Ginsenosides in Ginseng Formulation Granules by High Performance Liquid Chromatography-Mass Spectrometry
To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.Samples were extracted by 80%methanol with ultrasound extraction.The ginsenosides were separated on an Agilent Poroshell 120 EC-C18 column(2.1 mm×150 mm,2.7 μm)by gradient elution with 0.1%formic acid acetonitril and 0.1%formic acid water solution as mobile phase.A scheduled multiple reaction monitoring(sMRM)in negative ion mode was adopted in mass spectrometry acquisition.As a result,the linear correlation coefficients(r)of the 18 analytes in the linear ranges were more than 0.9970.The mean recoveries were all in the range of 94.5%～105.2%,and the relative standard deviations(RSD)were between 1.6%and 3.9%.Pse F11 was detected in 4 batches(12 batches in total)samples.The method was proved to be rapid,reliable and sensitive,which can be used to qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.
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