Comparative Study of Bacterial Enumeration Methods for Shale Gas Production Fluids
In view of the phenomenon that some oil and gas field service enterprises use plate counting method and ATP fluorescence rapid detection method instead of most probable number(MPN)to detect the bacterial content in shale gas production fluid,a comparative study was conducted on the determined results of the same sample using these three bacterial counting methods.The operation of plate counting method and the ATP fluorescence method is simpler than that of MPN method,but for shale gas production fluid,these two methods have the disadvantages of unclear detected bacterial types,inaccurate counting results and poor linear relationship.The bacteria detected by the plate counting method are mainly aerobic or oxygen-resistant saprophytic bacteria,and it is impossible to detect and identify sulfate-reducing bacteria closely related to corrosion.The ATP fluorescence method converts the total bacterial ATP content to obtain bacterial content.Currently,this method is more commonly used for rapid detection of restricted bacterial content in the medical and food industries,and there is no clear standard to rely on,and it cannot distinguish between dead and alive bacterial bodies.The MPN method can count the sulfate reducing bacteria(SRB),iron bacteria(IB)and saprophytic bacteria(TGB)related to corrosion by identifying the culture medium,and its detection limit is much lower than the other two methods.Therefore,it is more suitable for the detection of specific corrosion bacteria in shale gas production fluid.
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