血红蛋白封装UiO-66的制备及电化学性能
Preparation and Electrochemical Properties of Hemoglobin Encapsulated UiO-66
王建铭 1张子捷 1曾涵1
作者信息
- 1. 新疆师范大学,新疆 乌鲁木齐 830054
- 折叠
摘要
本文采用UiO-66 材料作为血红蛋白的锚定载体.制备Hb/UiO-66 电极,利用光谱学表征和电化学分析手段,深入研究Hb与 UiO-66 材料中各组分之间的相互作用,以及这些相互作用对分子内部的电子迁移和 H2O2 的电还原产生的影响,该体系成功实现了UiO-66固定化酶的直接电化学.Hb以物理吸附的方式包覆在UiO-66表面,研究表明Hb/UiO-66可以直接实现酶的电子迁移且电化学还原反应类型为表面控制型.灵敏度(S)和检出限(DL)分别为 8.3×10-4 A·L·mol-1和 3.18×10-8 mol/L,米氏常数(Km)为 17.96 μM.说明Hb保持着对底物H2O2较好的催化效果.
Abstract
In this paper,UiO-66 material was used as an anchoring carrier for haemoglobin.The Hb/UiO-66 electrode was prepared,and spectroscopic characterisation and electrochemical analysis were used to study in depth the interactions between Hb and the components in the UiO-66 material,and the effects of these interactions on the electron migration within the molecule and on the electroreduction of H2O2.The system successfully realised direct electrochemistry of the immobilised enzyme in UiO-66.The Hb was physically adsorbed and encapsulated in the Hb was physically adsorbed on the surface of UiO-66,and it was demonstrated that Hb/UiO-66 could directly achieve the electron migration of the enzyme and the type of electrochemical reduction reaction was surface-controlled.The sensitivity(S)and detection limit(DL)were 8.3×10-4 A·L·mol-1 and 3.18×10-8 mol/L,respectively,and the Mie constant(Km)was 17.96 μM,suggesting that Hb maintains a good catalytic effect on the substrate H2O2.
关键词
UiO-66/血红蛋白/电化学/相互作用/H2O2Key words
UiO-66/hemoglobin/electrochemical/interact/H2O2引用本文复制引用
基金项目
新疆师范大学化学重点学科规划项目(十三五)()
出版年
2024
NETL
NSTL
万方数据