ICP-MS Determination of Trace Calcium Content in HTK Solution
Objective:Based on inductively coupled plasmam assspectrometry(ICP-MS),a method for the determination of trace calcium in HTK solution was established.Methods:Dilute the HTK concentrate with 1%nitric acid,add the calcium standard solution to prepare a series of calcium standard solutions,select indium as the internal standard,use standard addition method and deduct blank for determination,and evaluate the precision,accuracy,and spiked recovery of the method.Result:The determination of calcium content showed a good linear relationship in the range of 0 mg/L to 1.5 mg/L(R=0.9999,n=7),with relative errors(RE)less than 2%,daily precision RSD less than 1%,and recovery rates of 95%to 105%.Conclusion:The method established in this study can well determine the calcium content in HTK solution,has a good linear range,and has good precision and accuracy.
histidine-tryptophan-ketoglutarate solutioncalcium ioninductively coupled plasma mass spectromet-rystandard addition methodmethods optimization
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