Mechanism of Cepharanthine against Rheumatoid Arthritis Based on Network Pharmacology
Objective:The aim of this study was to screen relevant targets and explore the potential mechanism of action of cepharanthine(CEP)for treating rheumatoid arthritis(RA)using network pharmacology.Methods:The study utilised the SwissTargetPrediction and DrugBank to predict potential targets for CEP.Additionally,the Disgenet,GeneCards,and NCBI were consulted to obtain RA-related targets.Potential targets for CEP and RA-related target intersections were then screened using Venny 2.1.The shared targets were imported into the STRING database to obtain the PPI network.The PPI network was visualised and screened for key genes using Cytoscape.Key genes were analysed for GO and KEGG enrichment using the DAVID database.Results:A total of 2196 targets related to RA were screened,along with 101 potential targets of action for CEP.56 common genes and 7 key genes were identified:JAK2,PRKCB,SRC,MTOR,AKT1,PI3K,and EGFR.The study conducted GO and KEGG enrichment analyses on key genes.The GO analysis revealed enrichment in the epidermal growth factor receptor signalling pathway,positive regulation of protein kinase B signalling,negative regulation of macroautophagy,positive regulation of nitric oxide biosynthesis process,and cell cycle protein-dependent protein serine/threonine kinase activity.The cytoplasm showed an enrichment of CC.Protein kinase activity,kinase activity,ATP binding,protein kinase C binding,protein serine/threonine kinase activity,and protein kinase binding were enriched in MF.KEGG was mainly enriched in the anticancer pathway,anti-inflammatory pathway,and antiviral pathway.Conclusion:this study provides a brief analysis of the potential targets and mechanisms of action of cepharanthine anti-RA.It offers a theoretical basis for further exploration of the potential protective mechanisms of cepharanthine anti-RA.
cepharanthinerheumatoid arthritisnetwork pharmacologymechanism of actionanti-inflammation
NETL
NSTL
万方数据
