Determination of Chlorphenamine Maleate in Compound Indomethacin Tincture by HPLC
Methods:The test was performed in WATERS C18 column(5 μm,4.6*250 mm)with acetonitrile and phosphate buffer solution(ammonium dihydrogen phosphate 11.5 g,dissolved in appropriate amount of water,added phosphoric acid 1 mL,diluted to 1000 mL by adding water)as mobile phase(80∶20).The flow rate was 1.0 mL/min,the detection wavelength was 262 nm,and the column temperature was 30℃.To establish the method for determination of Chlorphenamine Maleate in Compound indomethacin tincture by HPLC.Results:The linear range fell into 20.272～141.904 μg the calibration equation was y=0.1153x-0.0162(R=0.9999),and the average recovery was between 99.19%～101.68%with RSD as 0.9%for chlorphenamine maleate.The contents of Chlorphenamine Maleate 6 batches samples was between 98.97%～101.58%.Conclusion:This estabilished method is reliable and accurate.It can be used for determination of chlorphenamine maleate in Compound indomethacin.
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