Establishment of Quality Standard for Qinzhiyu Mixture
Objective:To establish the quality standard for Qinzhiyu mixture.Methods:Houttuyniae Herba,Glycayrrhizae Radix et Rhizoma and Scutellarie Radix were qualitatively identified by TLC method;the contents of baicalin and geniposide in Qinzhiyu mixture were deter mined by HPLC on a C18 analytical column(Wondasil,250 mm×4.6 mm,5 μm),and the mobile phase was composed of acetonitrile-0.1%formic acid solution with gradient elution,the column temperature was 30℃,the flow rate was 1.0 mL·min-1,the volume injection was 10 μL and the detection wavelengths were 283 nm(baicalin)and 238 nm(geniposide).Results:The TLC identification characteristics of Houttuyniae Herba,Glycayrrhizae Radix et Rhizoma and Scutellarie Radix were obvious and specific without negative interference;the linear range of baicalin and geniposide were 26.14～261.40 μg·mL-1(RSD=1.39%,n=6)and 20.40～204.00 μg·mL-1(RSD=1.10%,n=6);the average recovery was 99.50%(RSD=1.39%,n=6)and 98.97%(RSD=1.10%,n=6),respectively.The results of the methodological investigation showed that the contents of all index components met the requirements.Conclusion:The method has been established is simple,feasible and accurate,which can be used for the quality control of Qinzhiyu mixture.
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