Determination of Protein,Heavy Metal and GC-MS Analysis of Oil Extracted from Different Growth Stages of Periplaneta americana(L.)
In order to promote the comprehensive utilization of medicinal resources,the contents of protein and 4 heavy metal elements,as well as the composition of lipid soluble components,were tested in the 1st,3rd,6th instar nymphs,and adults of Periplaneta americana(L.).The content of crude protein was determined by kjeldahl method,the content of heavy metal was determined by ICP-MS,and the lipid soluble components and its relative content were determined by GC-MS.The crude protein content from Periplaneta americana(L.)at different growth stages was 56.61%to 67.38%;As≤0.271 4 mg/kg;Hg≤0.197 2 mg/kg;Pb≤0.548 2 mg/kg;Cd≤0.122 2 mg/kg;30 compounds were identified from oil extracts,with 5 components appear in the 4 growth stages.The protein content in adult Periplaneta americana(L.)reaches 63%,making it a high-quality source of feed protein.However,the mercury content exceeding the tolerance prescribed by hygienic quality of feed,so it is necessary to optimize the feed formula of Periplaneta americana(L.).The saturated fatty acids represented by palmitic acid are important factor responsible for the lipid toxicity of Periplaneta americana(L.),and the oil from it is rich in phenolic compounds with antioxidant activity.
Periplaneta americana(L.)GC-MScontentcrude proteinheavy metal